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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Knockdown of SLC41A1 magnesium transporter promotes mineralization and attenuates magnesium inhibition during osteogenesis of mesenchymal stromal cells

Fig. 3

Slc41a1 knockdown promoted mineralization and attenuated magnesium inhibition. a Slc41a1 expression of undifferentiated mMSCs after Slc41a1 gene knockdown (N = 3, n = 3). b, c Alizarin Red S staining (left) and the quantification (right) of wild-type mMSCs (ctrl) and mMSCs with Slc41a1 gene knockdown (KD) 6 and 12 days after osteogenic induction with normal (N: 0.8 mM) and high (H: 5.8 mM) extracellular magnesium concentration (N = 3, n = 3). d Images of mMSCs with Slc41a1 gene knockdown before and 3 and 6 days after osteogenic induction with normal magnesium concentration. Scale bar: 100 μm. e Gene expression of undifferentiated mMSCs with Slc41a1 gene knockdown and 3 and 6 days after osteogenic induction (D0, D3, D6) under 0.8 and 5.8 mM magnesium concentration. Data normalized by the gene expression of undifferentiated Slc41a1-knockdown MSCs (D0) and presented as mean ± SEM (N = 3, n = 3) (* p< 0.05)

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Stem Cell Research & Therapy

ISSN: 1757-6512