Fig. 2

Scanning electron microscopy, DNA content analysis, and proliferation assay. Electron micrographs of the mucosal (b and d) and serosal (a and c) sides of fresh SIS (a and b) and 5% PAA-treated SIS (c and d). A high level of porosity and large pore size were evident after treatment with 5% PAA. Scale bar = 60 μm. The DNA content analysis (e) showed a decrease in DNA content after treatment with 5% PAA. A significant difference in the DNA content was observed between the two groups (*P < 0.05). f Analysis of cell growth. The OD values in the scaffold groups were similar to those in the control group (P > 0.05). d days, OD optical density, PAA peracetic acid, SIS small intestinal submucosa