Pulp regeneration by transplantation of dental pulp stem cells in pulpitis: a pilot clinical study

Table 2 Cell biological characteristics, including viability, expression rate of stem cell markers, cell survival rate, and karyotype

Cell characteristics	Patient 1	Patient 2	Patient 3	Patient 4	Patient 5	Average
Total cell product number × 10⁶	1.4^a	10.3	17.2	19.6	14.8	15.5 ± 4.0^b
Viability (%)	80.5	93.7	77.3	85.0	78.3	83.0 ± 6.7
Stem cell markers (%)
CD29	97.0	99.6	97.7	99.6	99.5	98.7 ± 1.2
CD44	99.8	99.4	99.2	99.8	99.1	99.5 ± 0.3
CD105	80.4	99.9	97.0	98.4	95.6	94.3 ± 7.9
CD31	0.9	0.9	0.9	0.0	0.5	0.6 ± 0.4
Karyotype	46,XX (17/20) 45,X (2/20) 45,X,-9 (1/20)	46,XY (20/20)	47,XYY (20/20)	46,XY (19/20) 45,X (1/20)	46,XX (20/20)

^a The mobilized dental pulp stem cells (MDPSCs) of patient 1 were damaged at passage 4 of culture due to the high temperature of the incubator since the air-conditioner was broken in the Cell Processing Center, and the cryopreserved MDPSCs at passage 3 were used for further expansion, resulting in a smaller number of cells at passage 7. Karyotype was analyzed at passage 10
^b Excluding patient 1

ISSN: 1757-6512