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Table 1 Flow cytometry with Annexin V and PI for analysis of necrotic and apoptotic processes in the cells after the studied cryopreservation procedures (mean ± SD)

From: Influence of temperature fluctuations during cryopreservation on vital parameters, differentiation potential, and transgene expression of placental multipotent stromal cells

  FACS Anexin V with PI
Anexin V–,PI– (no necrosis or apoptosis), % Anexin V–,PI + (necrosis), % Anexin V+,PI– (apoptosis), % Anexin V+,PI + (necrosis and apoptosis), %
Constant cryopreservation temperature, –196 °C 83.88 ± 3.7 1.02 ± 0.05 1.33 ± 0.89 13.7 ± 1.7
Temperature fluctuations between –196 °C and –80 °C 5 cycles 82.7 ± 2.1 1.19 ± 0.03 2.21 ± 0.8* 14.66 ± 2.0
10 cycles 68.2 ± 2.1* 1.12 ± 0.02 2.1 ± 0.09* 27.98 ± 2.2*
20 cycles 56.8 ± 1.5* 0.54 ± 0.01 2.5 ± 0.08* 41.47 ± 3.82*
30 cycles 54.2 ± 3.1* 1.01 ± 0.02 3.05 ± 0.09* 42.68 ± 2.09*
40 cycles 52.5 ± 1.8* 0.72 ± 0.02 2.65 ± 0.15* 43.64 ± 1.28*
50 cycles 51.8 ± 1.9* 0.86 ± 0.01 1.83 ± 0.05* 46.22 ± 2.38*
Temperature fluctuations between –196 °C and –100 °C 5 cycles 81.6 ± 2.8 0.48 ± 0.02 1.67 ± 0.06 16.09 ± 1.44
10 cycles 80.2 ± 2.9 0.58 ± 0.02 2.17 ± 0.07* 17.47 ± 1.88*
20 cycles 72.0 ± 2.5* 0.89 ± 0.01 2.03 ± 0.02* 26.05 ± 3.54*
30 cycles 70.6 ± 3.1* 1.18 ± 0.02 2.96 ± 0.05* 24.11 ± 4.37*
40 cycles 64.8 ± 2.4* 0.65 ± 0.04 3.23 ± 0.01* 25.96 ± 2.78*
50 cycles 59.6 ± 3.3* 1.6 ± 0.01 1.15 ± 0.01 38.94 ± 3.64*
Temperature fluctuations between –196 °C and –150 °C 5 cycles 88.37 ± 2.7 0.55 ± 0.01 1.94 ± 0.01 11.82 ± 3.02
10 cycles 83.6 ± 1.8 0.83 ± 0.07 1.8 ± 0.09 13.64 ± 1.1
20 cycles 80.02 ± 3.2 0.57 ± 0.06 1.59 ± 0.03 17.68 ± 2.81
30 cycles 78.79 ± 4.5* 0.34 ± 0.02 1.57 ± 0.03 19.30 ± 0.77*
40 cycles 70.39 ± 4.1* 0.98 ± 0.02 1.3 ± 0.03 25.46 ± 3.39*
50 cycles 64.49 ± 3.6* 0.96 ± 0.08 1.6 ± 0.05 27.74 ± 2.7*
  1. *p < 0.05, versus constant cryopreservation temperature control
  2. PI propidium iodide