Fig. 2

Stroke does not affect differentiation capacity of human skin-derived neural progenitors transplanted adjacent to SVZ. a-b Fluorescence photomicrographs showing grafted cells (GFP+, green) co-expressing the mature neuron-specific marker NeuN (red) and Hoechst counterstain (blue) 2 months after transplantation in intact (a) and stroke-subjected (MCAO, b) rats. Arrows depict grafted NeuN+ cells while arrowheads depict host NeuN+ cells. c-d Percentage of NeuN+ cells (c) and GFAP+ area (d) in the grafts from intact (n = 6) and stroke-injured (n = 7) rats. Data represent means ± SEM. Fluorescence photomicrographs showing grafted cells (GFP+, green) co-expressing human-specific GFAP (in red) 2 months after transplantation in intact (e) and stroke-injured (MCAO, f) rats. Arrows depict grafted GFAP+ cells. g Percentage KGA+ area (glutamatergic neuron marker) in grafts from intact (n = 6) and stroke-subjected (n = 7) rats. Data represent means ± SEM. h -i Fluorescence photomicrographs showing grafted cells (GFP+, green) co-expressing KGA (in red) 2 months after transplantation in intact (h) and stroke-subjected (MCAO, i) rats. Scale bars represent 25 μm