Fig. 2
From: Isolation and characterization of equine native MSC populations
Isolation of CD34+ and CD146+ cells from adipose SVF. a, b Events displayed as FSC-A vs FSC-H to select singlets (a) were further gated to exclude cell fragments or noncellular material (b). c–e Sequence of plots showing selection of DAPI-negative, CD144(AF405)-negative, and CD45(FITC)-negative cells to obtain live, endothelial-negative, and hematopoietic-negative fractions, respectively. (c1–c4) Dot-plots for CD144 (c1, c2) and CD45 (c3, c4) controls showing unstained cells (c1, c3) and secondary antibody conjugated to AF405 (c2) or isotype conjugated to FITC (c4). f Double-plot displaying CD34+ (Q1) and CD146+ (Q4) cell subpopulations and respective isotype controls (c5) conjugated to PE and AF647, respectively. Filters used: 450/50 for AF405, 525/50 for FITC, 586/15 for PE, and 670/30 for AF647. FSC-A forward scatter area, FSC-H forward scatter height, SSC-A side scatter area