Table 3 Effect of the addition of empty microcarriers at seeding on cell density (number of cells per microcarrier and per cm2) as well as aggregate size (number of microcarriers per construct)
Fold reduction in cell density | 0a | 0.25 | 0.50 |
|---|---|---|---|
Cells per construct | 10 × 103 | 10 × 103 | 10 × 103 |
Cytodex 1 microcarriers (mg) per construct | 0.0713 | 0.0886 | 0.1069 |
Cells per mg of microcarriers (cells/mg) | 1.40 × 105 | 1.13 × 105 | 9.35 × 104 |
Cytodex 1 microcarriers (number) per construct | 307 | 381 | 460 |
Cells per microcarrier (cells) | 32.6 | 26.3 | 21.8 |
Surface area of Cytodex 1 microcarriers (cm2) per construct | 0.314 | 0.390 | 0.470 |
Cells per cm2 (cells/cm2) | 31,885 | 25,663 | 21,256 |