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Fig. 2 | Stem Cell Research & Therapy

Fig. 2

From: Basic fibroblast growth factor reduces scar by inhibiting the differentiation of epidermal stem cells to myofibroblasts via the Notch1/Jagged1 pathway

Fig. 2

bFGF inhibited differentiation of ESCs to MFB by activating the Notch1/Jagged1 pathway in vitro. a Representative immunoblot and results of densitometric analysis of blots showing relative protein levels of Jagged1 in control, negative control (ESCs transfected with a scrambled siRNA for Jagged1), and siJagged1 (ESCs transfected with siRNA specific for Jagged1). GAPDH used as a loading control. b Representative qRT-PCR analysis showing relative mRNA levels of Jagged1 in control, negative control, and siJagged1. c Representative immunoblot and results of densitometric analysis of blots showing relative protein levels of α-SMA, Col I, and Col III for each group on day 10. d Representative immunoblot and results of densitometric analysis of blots showing relative protein levels of Jagged1, Notch1, and Hes1 for each group on day 10. e Representative qRT-PCR analysis showing relative mRNA levels of α-SMA, Col I, and Col III for each group on day 10. f Representative qRT-PCR analysis showing relative mRNA levels of Jagged1, Notch1, and Hes1 for each group on day 10. a, b *P < 0.05. d–f Error bars represent SEM. Student’s t test, *P < 0.05 compared with control value (n = 5), #P < 0.05 compared with bFGF value (n = 5). Con control NC negative control, bFGF basic fibroblast growth factor, Col collagen

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Stem Cell Research & Therapy

ISSN: 1757-6512