Fig. 3

A high concentration of IFN-γ decreases bone-related protein mRNA expression in C-MSC. a-d C-MSCs were treated with or without various doses of IFN-γ (10, 50, 100 ng/mL) for 24 h. The plot shows the ratio of OPN, OC, ALPase, and BMP-2 mRNAs to GAPDH mRNA. Values represent means ± S.D. of four cultures. Values represent means ± S.D. of four cultures. ^* p < 0.05, ^** p < 0.01: values differ significantly (t test). Similar results were obtained from three experiments. ALPase alkaline phosphatase, BMP bone morphogenic protein, GAPDH glyceraldehyde-3-phosphate dehydrogenase, IFN interferon, OC osteocalcin, OPN osteopontin