Table 1 Sense primers and antisense primers for real-time PCR
Target gene | Primer sequence | |
|---|---|---|
IDO | Forward | 5′-CAAAGGTCATGGAGATGTCC-3′ |
Reverse | 5′-CCACCAATAGAGAGACCAGG-3′ | |
ALPase | Forward | 5′-GCGGTGAACGAGAGAATG-3′ |
Reverse | 5′-CGTAGTTCTGCTCGTGCAC-3′ | |
BMP-2 | Forward | 5′-CTGTATCGCAGGCACTCA-3′ |
Reverse | 5′-CTCCGTGGGGATAGAACTT-3′ | |
OC | Forward | 5′-GCAGCGAGGTAGTGAAGAGAC-3′ |
Reverse | 5′-GGTCAGCCAACTCGTCACAG-3′ | |
OPN | Forward | 5′-GATGGCCGAGGTGATAGTGT-3′ |
Reverse | 5′-CCATTCAACTCCTCGCTTTC-3′ | |
GAPDH | Forward | 5′-AACGTGTCAGTGGTGGACCTG-3′ |
Reverse | 5′-AGTGGGTGTCGCTGTTGAAGT-3′ |