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Table 1 Sense primers and antisense primers for real-time PCR

From: Xenotransplantation of interferon-gamma-pretreated clumps of a human mesenchymal stem cell/extracellular matrix complex induces mouse calvarial bone regeneration

Target gene   Primer sequence
IDO Forward 5′-CAAAGGTCATGGAGATGTCC-3′
  Reverse 5′-CCACCAATAGAGAGACCAGG-3′
ALPase Forward 5′-GCGGTGAACGAGAGAATG-3′
  Reverse 5′-CGTAGTTCTGCTCGTGCAC-3′
BMP-2 Forward 5′-CTGTATCGCAGGCACTCA-3′
  Reverse 5′-CTCCGTGGGGATAGAACTT-3′
OC Forward 5′-GCAGCGAGGTAGTGAAGAGAC-3′
  Reverse 5′-GGTCAGCCAACTCGTCACAG-3′
OPN Forward 5′-GATGGCCGAGGTGATAGTGT-3′
  Reverse 5′-CCATTCAACTCCTCGCTTTC-3′
GAPDH Forward 5′-AACGTGTCAGTGGTGGACCTG-3′
  Reverse 5′-AGTGGGTGTCGCTGTTGAAGT-3′
  1. ALPase alkaline phosphatase, BMP bone morphogenic protein, GAPDH glyceraldehyde-3-phosphate dehydrogenase, IDO indoleamine 2,3-dioxygenease, OC osteocalcin, OPN osteopontin