Fig. 2
From: High quality clinical grade human embryonic stem cell lines derived from fresh discarded embryos
Immunostaining of MAN lines 10–16 for pluripotency markers. At approximately p10 after 5 days of culture on human dermal fibroblasts (hDFs), positive pluripotency markers (green) were assessed including OCT4, Nanog, SOX2, SSEA4 and TRA160. The differentiation marker SSEA1 was used as a negative control with DAPI (blue) as a counterstain. Isotype controls were mouse IgG, mouse IgM and rabbit IgG. Scale bars represent 100 μM