|
Gene
|
Primer
|
Sequence (5′–3′)
|
|---|
|
ACAN
|
Forward
|
ACCAGACTGTCAGATACCCC
|
|
Reverse
|
CATAAAAGACCTCACCCTCC
|
|
SOX-9
|
Forward
|
GCCTCTACTCCACCTTCACC
|
|
Reverse
|
GTAGACGGGTTGTTCCCAGT
|
|
COL2A1
|
Forward
|
ATTGCCTATCTGGACGAAGC
|
|
Reverse
|
GCAGTGTACGTGAACCTGCT
|
|
CA12
|
Forward
|
CGTGCTCCTGCTGGTGATCT
|
|
Reverse
|
AGTCCACTTGGAACCGTTCACT
|
|
KRT19
|
Forward
|
AAGACACACTGGCAGAAACG
|
|
Reverse
|
GATTCTGCCGCTCACTATCA
|
|
HIF-1α
|
Forward
|
GCCAGACGATCATGCAGCTA
|
|
Reverse
|
ATCCATTGATTGCCCCAGCA
|
|
MMP-1
|
Forward
|
GGAACAGATACGAAGAGGAAACA
|
|
Reverse
|
TGTGGGAATCAGAGGTAGAAGA
|
|
MMP-3
|
Forward
|
GCATTGGCTGAGTGAAAGAGAC
|
|
Reverse
|
ATGATGAACGATGGACAGATGA
|
|
TIMP-1
|
Forward
|
GGTTCCCTGGCATAATCTGAG
|
|
Reverse
|
ATCGCTCTGGTAGCCCTTCT
|
|
β-ACTIN
|
Forward
|
GTGGGGCGCCCCAGGCACCA
|
|
Reverse
|
CTTCCTTAATGTCACGCACGATTTC
|
-
ACAN aggrecan, COL2A1 collagen II α1, SOX-9 SRY-related high mobility group-box gene 9, KRT19 cytokeratin-19, CA12 carbonic anhydrase XII, HIF-1α hypoxia-inducible factor 1α, MMP matrix metalloproteinase, TIMP-1 tissue inhibitor of metalloproteinases-1
