Table 1 Quantitative real-time PCR primers
Gene | Primer | Sequence (5′–3′) |
|---|---|---|
ACAN | Forward | ACCAGACTGTCAGATACCCC |
Reverse | CATAAAAGACCTCACCCTCC | |
SOX-9 | Forward | GCCTCTACTCCACCTTCACC |
Reverse | GTAGACGGGTTGTTCCCAGT | |
COL2A1 | Forward | ATTGCCTATCTGGACGAAGC |
Reverse | GCAGTGTACGTGAACCTGCT | |
CA12 | Forward | CGTGCTCCTGCTGGTGATCT |
Reverse | AGTCCACTTGGAACCGTTCACT | |
KRT19 | Forward | AAGACACACTGGCAGAAACG |
Reverse | GATTCTGCCGCTCACTATCA | |
HIF-1α | Forward | GCCAGACGATCATGCAGCTA |
Reverse | ATCCATTGATTGCCCCAGCA | |
MMP-1 | Forward | GGAACAGATACGAAGAGGAAACA |
Reverse | TGTGGGAATCAGAGGTAGAAGA | |
MMP-3 | Forward | GCATTGGCTGAGTGAAAGAGAC |
Reverse | ATGATGAACGATGGACAGATGA | |
TIMP-1 | Forward | GGTTCCCTGGCATAATCTGAG |
Reverse | ATCGCTCTGGTAGCCCTTCT | |
β-ACTIN | Forward | GTGGGGCGCCCCAGGCACCA |
Reverse | CTTCCTTAATGTCACGCACGATTTC |