Fig. 1

Characterization of TIC cultures derived from human MPMs. a Morphology of MM1, MM3, and MM4 TICs maintained in stem cell-permissive conditions: upper panels, sphere-forming cells derived from xenografted tumors in mice and grown as nonadherent clusters (scale bar 20 μm); lower panels, representative images of monolayers obtained by spheres (scale bar 100 μm). b Histological sections of tumors recovered from mice xenografted with MM1, MM3, and MM4 cells stained with the indicated antibodies. Immunoreactivity was detected by immunohistochemistry and immunofluorescence. Representative images are reported (scale bar 200 μM). c MM1, MM3, and MM4 cells, recovered from primary xenografts, analyzed by FACS for the expression of the indicated molecules. Green, cells stained with appropriate PE or FITC-conjugated antibodies; gray, cells incubated with PE or FITC-conjugated isotype-specific control antibody (Color figure online)