Fig. 2

Effect of ADHLSCs on HSC proliferation. Cell cycle analysis of PI-stained HSCs demonstrated an increase in the number of HSC blocked in G0/G1 phase and a decrease in the number of HSCs in G2/M phase after 24 h of co-culture at an ADHLSC:HSC ratio of 1:100 (a) and after 24 h of incubation with ADHLSC-conditioned medium (b) (N = 4). c The Ki67 immunostaining of HSCs showed a significant decrease in the number of immunostained nuclei of HSCs pre-incubated for 24 h with ADHLSC conditioned medium (N = 4). For each experimental condition, four different fields were analyzed, and a total of 2500 nuclei were counted using Image J software. Results are expressed as mean ± standard error of the mean (SEM). ^*** p value < 0.001; ^** p < 0.01; ^* p < 0.05. ADHLSCs adult-derived human liver mesenchymal stem/progenitor cells, HSC hepatic stellate cells