Table 1 Sequence of the oligonucleotides for real-time PCR
Gene | Sequence (5′ → 3′) | |
|---|---|---|
β-actin | F | GTGGGGCGCCCCAGGCACCA |
R | CTTCCTTAATGTCACGCACGATTTC | |
Runx-2 | F | ACGACAACCGCACCATGGT |
R | CTGTAATCTGACTCTGTCCT | |
ALP | F | TGGAGCTTCAGAAGCTCAACACCA |
R | ATCTCGTTGTCTGAGTACCAGTCC | |
Collagen I | F | CCTGAGCCAGCAGATTGA |
R | TCCGCTCTTCCAGTCAG | |
BMP-2 | F | GAGGTCCTGAGCGAGTTCGA |
R | ACCTGAGTGCCTGCGATACA | |