Fig. 2

Bone marrow mononuclear cells (BMNCs) definitively contribute to de novo muscle fiber regeneration, but do not promote transcriptional or functional gains beyond those provided by minced muscle graft (MG). a Representative hematoxylin and eosin sections and immunofluorescence staining of green fluorescent protein (GFP)⁺ muscle fibers in the defect regions of 50% MG + BMNC (right) and 50% MG treated VML injuries (left) in wild-type hosts at 8 weeks post-injury. b Transcription of myogenesis markers, embryonic myosin heavy chain (eMHC) and myogenin, was not significantly different between BMNC supplemented and unsupplemented MG repair groups for either of the time points investigated (p > 0.05). c BMNC contribution to myofiber regeneration did not result in a change in in vivo isometric tetanic force generation of treated TA muscles at 8 weeks post-injury for any of the flexion angles tested. Values are shown as means ± SEM. *p < 0.05, 50% MG and 50% MG + BMNC groups versus contralateral limb