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Table 1 Observed changes in immunogenic and immunomodulatory molecules after 3 days in vitro treatment of ucMSC

From: Cytokine treatment optimises the immunotherapeutic effects of umbilical cord-derived MSC for treatment of inflammatory liver disease

  Immunogenic read out parameters Immunomodulatory read out parameters Fold increase of gene expression compared to unstimulated
HLA class I- expressing cells [%] HLA class II- expressing cells [%] L-Kynurenine (correlated IDO activity) [μM] PGE2 [ng/ml] PD-L1- expressing cells [%] CD73-expressing cells [%] IL-1RA [corrected for GAPDH]
[-] 7 ± 1 10 ± 3 2 ± 5 14 ± 3 46 ± 6 95 ± 2 1 ± 0
IFN-y 60 ± 9* 68 ± 9* 27 ± 10* 1 ± 0* 95 ± 3* 94 ± 3 1 ± 0
IFN-β 36 ± 12* 14 ± 5 10 ± 10 3 ± 2 73 ± 12 91 ± 5 4 ± 1
TGFβ 9 ± 3 10 ± 3 3 ± 1 3 ± 2 58 ± 7 89 ± 5 4 ± 4
Starv 15 ± 3* 4 ± 2 6 ± 3 4 ± 3 24 ± 8* 100 ± 0 1 ± 0
VitB6 47 ± 5* 34 ± 9* 3 ± 5 12 ± 5 59 ± 14 100 ± 0 67 ± 42
Starv + VitB6 62 ± 7* 22 ± 5* 0 ± 3 6 ± 3 72 ± 7* 100 ± 0 507 ± 262
RA 26 ± 5* 18 ± 5 0 ± 0 5 ± 2 59 ± 11 98 ± 1 5 ± 4
MC 85 ± 4* 76 ± 7* 29 ± 8* 8 ± 3 97 ± 1* 98 ± 2 3 ± 2
  1. The table displays the mean ± SEM percentages of MSC-expressing surface markers HLA class I and II, PD-L1 and CD73, measured by flow cytometry , the mean ± SEM concentration of L-Kynurenine [μM] and PGE2 [ng/ml] measured by ELISA and fold increases in IL1RA mRNA expression compared to unstimulated MSC. n = 5
  2. ucMSC umbilical cord-derived mesenchymal stromal cells, HLA human leukocyte antigen, IDO indoleamine 2,3 dioxygenase, PGE 2 prostaglandin E2, PD-L1 programmed cell death ligand 1, IL interleukin, IFN-γ interferon gamma, IFN-β interferon beta, TGFβ transforming growth factor beta, Starv starvation, VitB 6 Vitamin B6, pyridoxal hydrochloride, RA retinoic acid, MC multiple cytokine combination
  3. *Indicates p < 0.05