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Table 1 Observed changes in immunogenic and immunomodulatory molecules after 3 days in vitro treatment of ucMSC

From: Cytokine treatment optimises the immunotherapeutic effects of umbilical cord-derived MSC for treatment of inflammatory liver disease

 

Immunogenic read out parameters

Immunomodulatory read out parameters

Fold increase of gene expression compared to unstimulated

HLA class I- expressing cells [%]

HLA class II- expressing cells [%]

L-Kynurenine (correlated IDO activity) [μM]

PGE2 [ng/ml]

PD-L1- expressing cells [%]

CD73-expressing cells [%]

IL-1RA [corrected for GAPDH]

[-]

7 ± 1

10 ± 3

2 ± 5

14 ± 3

46 ± 6

95 ± 2

1 ± 0

IFN-y

60 ± 9*

68 ± 9*

27 ± 10*

1 ± 0*

95 ± 3*

94 ± 3

1 ± 0

IFN-β

36 ± 12*

14 ± 5

10 ± 10

3 ± 2

73 ± 12

91 ± 5

4 ± 1

TGFβ

9 ± 3

10 ± 3

3 ± 1

3 ± 2

58 ± 7

89 ± 5

4 ± 4

Starv

15 ± 3*

4 ± 2

6 ± 3

4 ± 3

24 ± 8*

100 ± 0

1 ± 0

VitB6

47 ± 5*

34 ± 9*

3 ± 5

12 ± 5

59 ± 14

100 ± 0

67 ± 42

Starv + VitB6

62 ± 7*

22 ± 5*

0 ± 3

6 ± 3

72 ± 7*

100 ± 0

507 ± 262

RA

26 ± 5*

18 ± 5

0 ± 0

5 ± 2

59 ± 11

98 ± 1

5 ± 4

MC

85 ± 4*

76 ± 7*

29 ± 8*

8 ± 3

97 ± 1*

98 ± 2

3 ± 2

  1. The table displays the mean ± SEM percentages of MSC-expressing surface markers HLA class I and II, PD-L1 and CD73, measured by flow cytometry , the mean ± SEM concentration of L-Kynurenine [μM] and PGE2 [ng/ml] measured by ELISA and fold increases in IL1RA mRNA expression compared to unstimulated MSC. n = 5
  2. ucMSC umbilical cord-derived mesenchymal stromal cells, HLA human leukocyte antigen, IDO indoleamine 2,3 dioxygenase, PGE 2 prostaglandin E2, PD-L1 programmed cell death ligand 1, IL interleukin, IFN-γ interferon gamma, IFN-β interferon beta, TGFβ transforming growth factor beta, Starv starvation, VitB 6 Vitamin B6, pyridoxal hydrochloride, RA retinoic acid, MC multiple cytokine combination
  3. *Indicates p < 0.05
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Stem Cell Research & Therapy

ISSN: 1757-6512