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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Engineering biomimetic periosteum with β-TCP scaffolds to promote bone formation in calvarial defects of rats

Fig. 3

The view and microscopic morphology of UM (a, b) and OM (e, f), which show the cell sheets formed multiple layers and can be lifted up by point forceps; when iECs were seeded in the UM at day 7 (c), the UM morphology is significantly changed. Immunofluorescent staining of CD31 showed the in vitro angiogenesis of the prevascularized cell sheet (iECs/UM) (d). ALP at day 14 (g) and alizarin red staining at day 21 (h) on the OM sheet show obvious osteogenic differentiation characteristics of the OM sheet. Scale bars = 50 μm (b,c,f) and 20 μm (d)

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Stem Cell Research & Therapy

ISSN: 1757-6512