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Fig. 6 | Stem Cell Research & Therapy

Fig. 6

From: Effects of mechanical stimulation on the reprogramming of somatic cells into human-induced pluripotent stem cells

Fig. 6

Characterization of iPSCs. a Confirmation of exogenous OCT4, SOX2, KLF4, and c-MYC transduction. b Expression of iPSC-related markers at passage 4 (OCT4, SOX2, NANOG, TERT, and TDGF). c Confirmation of the differentiability of iPSCs by immunostaining for OCT4, SOX2, NANOG, ALP, and SSEA. Scale bar = 100 μm. d Formation of embryonic bodies at passage 4. e, f Morphology of spontaneously differentiated cells after being cultured on dishes coated with 0.1% gelatin. Scale bar = 100 μm. g Expression of related markers to confirm three germ-layered differentiation: SOX17, AFP, CK8, CK18, PAX, and MAP2. Confirmation of loss of stem cell markers in spontaneously differentiated cells. ALP alkaline phosphatase, CP commercial plates, D days, EB embryoid body, Exo exogenous, FM flexible membranes, GFP green fluorescent protein, iPSC induced pluripotent stem cell, Retro-OSKM pMXs-OCT4, pMXs-SOX2, pMXs-KLF, pMXs-c-MYC

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