Table 1 Primer sequences to amplify cDNA using real time polymerase chain reaction
Genes | Primers | Primer squences | Primer Size (bp) | Tm | Product size (bp) |
|---|---|---|---|---|---|
MMP-1 | Forward Primer | 5′-AAGGCCAGTATGCACAGCTT-3′ | 20 | 58.3 | 480 |
Reverse Primer | 5′-TGCTTGACCCTCAGAGACCT-3′ | 20 | |||
MMP-2 | Forward Primer | 5′-TTTCCATTCCGCTTCCAGGGCAC-3′ | 23 | 63 | 253 |
Reverse Primer | 5′-TCGCACACCACATCTTTCCGTCACT-3′ | 25 | |||
MMP-3 | Forward Primer | 5′-GGCTTTCCCAAGCAAATAGC-3′ | 20 | 57.3 | 205 |
Reverse Primer | 5′-GTGCCCATATTGTGCCTTCT-3′ | 20 | |||
MMP-7 | Forward Primer | 5′-TCCAACCTATGGAAATGGAGA-3′ | 21 | 57.3 | 196 |
Reverse Primer | 5′-GGAGTGGAGGAACAGTGCTT-3′ | 20 | |||
MMP-8 | Forward Primer | 5′-TCTGCAAGGTTATCCCAAGG-3′ | 20 | 57.3 | 154 |
Reverse Primer | 5′-ACCTGGCTCCATGAATTGTC-3′ | 20 | |||
MMP-9 | Forward Primer | 5′-CCTGCCAGTTTCCATTCATC-3′ | 20 | 57.3 | 455 |
Reverse Primer | 5′-GCCATTCACGTCGTCCTTAT-3′ | 20 | |||
MMP-12 | Forward Primer | 5′-ACAGATGATGGACCCTGGTT-3′ | 20 | 57.3 | 392 |
Reverse Primer | 5′-AGAGTCAAGCAAGAATGGACAA-3′ | 22 | |||
MMP-13 | Forward Primer | 5′-AACATCCAAAAACGCCAGAC-3′ | 20 | 55.3 | 166 |
Reverse Primer | 5′-GGAAGTTCTGGCCAAAATGA-3′ | 20 | |||
MMP-15 | Forward Primer | 5′-AGGAGACACAGCGTGGAGAC-3′ | 20 | 60 | 514 |
Reverse Primer | 5′-TTGCAGTAAAGCAGGACACG-3′ | 20 | |||
MMP-16 | Forward Primer | 5′-GACATGCTCTGGGATTGGAG-3′ | 20 | 57.3 | 217 |
Reverse Primer | 5′-TCATTTTTCCTTGGGTCAGC-3′ | 20 | |||
MMP-24 | Forward Primer | 5′-GAACCTGTGGGCAAGACCTA-3′ | 20 | 58.3 | 213 |
Reverse Primer | 5′-TGACAACCAGAAACTGAGCG-3′ | 20 |