Fig. 2

Quantitative FACS measurement of mitochondrial transfer. a Flow cytometry analysis was performed after 48, 72 and 96 h of co-culture. Co-cultures between MSCs and fibroblasts and between fibroblasts and fibroblasts (e.g. healthy controls with NDUFS4-deficient cell lines) were measured. b Mitochondrial transfer during different culture conditions/drug treatment. mMSC mouse mesenchymal stem cell, MSC mesenchymal stem cell, Gal galactose medium, BSO buthioninesulfoximine, 2DG 2-deoxy-d-glucose, TNF-α tumour necrosis factor alpha, NDUFS4 NADH dehydrogenase ubiquinone Fe-S protein 4, WT wild type. Data shown as mean (% of “mito-positive” cells per measurement) ± SD. *p < 0.05, **p < 0.01, ***p < 0.001