Fig. 4

CNBP and NEDD1 gene expression in hES and somatic cells. Gene expression analysis by quantitative PCR of the top two genes found overlapped between our microarray data, comparing control and H₂O₂-treated HUES3 cells, and published transcriptome data of human oocytes and seven pre-implantation embryo stages: zygote, 2-cell, 4-cell, 8-cell, morula and blastocyst. Dark grey columns represent HUES3 cells and black columns represent HUES7 cells. Relative gene expression between CTR untreated cells and 4, 8 and 16 μM 72 h H₂O₂ treatment for CNBP gene (a) and NEDD1 gene (c). White columns represent Hs27 cells and striped columns represent HUVEC. Relative gene expression between CTR untreated somatic control cells, and 4, 8, 16, 32 and 64 μM conditions for fibroblast, and also 128 and 256 μM H₂O₂ conditions for endothelial cells, of 72 h H₂O₂ treatment for CNBP gene (b) and NEDD1 gene (d). Three qRT-PCR analyses were conducted with each of three independent biological replicates and the data were analysed using a two-sample Student’s t test. Columns identified with an asterisk are significantly different from untreated control, p ≤0.05