Cell sex affects extracellular matrix protein expression and proliferation of smooth muscle progenitor cells derived from human pluripotent stem cells

Table 1 Primers used for real-time quantitative reverse transcription-polymerase chain reaction

Gene	Strand	5′–3′ sequence	Accession number
GAPDH	Sense	CTCAACGACCACTTTGTCAAGCTCA	NC_000012.12
GAPDH	Anti-sense	GGTCTTACTCCTTGGAGGCCATGTG	NC_000012.12
Hu-SMA-alpha	Sense	CCAGTGTGGAGCAGCCCAGC	NC_000001.11
Hu-SMA-alpha	Anti-sense	TCACCCCCTGATGTCTGGGACG	NC_000001.11
Hu-Smoothelin	Sense	TTGGACAAGATGCTGGATCA	NC_000022.11
Hu-Smoothelin	Anti-sense	CGCTGGTCTCTCTTCCTTTG	NC_000022.11
SM22-alpha	Sense	GCTTGGAGCCATCAGGGTA	NC_000011.10
SM22-alpha	Anti-sense	GGAGTGGATCATAGTGCAGTGT	NC_000011.10
Collagen I-alpha1	Sense	TGTCTTATGGCTATGATGAG	NC_000017.11
Collagen I-alpha1	Anti-sense	ATCCAAACCACTGAAACC	NC_000017.11
Collagen III-alpha1	Sense	GGCTCCTGGTGAGCGAGGAC	NC_000002.12
Collagen III-alpha1	Anti-sense	CCCATTTGCACCAGGTTCTCC	NC_000002.12
Hu-Elasin	Sense	GTCGCAGGTGTCCCTAGTGT	NC_000007.14
Hu-Elasin	Anti-sense	GGTCCCCACTCCGTACTTG	NC_000007.14
TIMP-1	Sense	GGGCTTCACCAAGACCTACA	NC_000023.11
TIMP-1	Anti-sense	TGCAGGGGATGGATAAACAG	NC_000023.11
MMP-1	Sense	AGTGACTGGGAAACCAGATGCTGA	NC_000011.10
MMP-1	Anti-sense	TCTGCTTGACCCTCAGAGACC	NC_000011.10
MMP-2	Sense	CGAATCCATGATGGAGAGGC	NC_000016.10
MMP-2	Anti-sense	TCCGTCCTTACCGTCAAAGG	NC_000016.10

GenBank accession numbers indicate transcript variants with homologous sequences to primers

ISSN: 1757-6512