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Fig. 4 | Stem Cell Research & Therapy

Fig. 4

From: Skeletal muscle-derived interstitial progenitor cells (PICs) display stem cell properties, being clonogenic, self-renewing, and multi-potent in vitro and in vivo

Fig. 4

PIC sub-clone characterisation. a Representative light microscope images of bulk cultured, clonal, and sub-clonal PICs. b Sub-cloning efficiency of C9 PICs at every fifth passage up to passage (P)20. Data are mean ± SD; n = 3. Sca-1 (c) and PW1 (d) expression quantified by flow cytometry for bulk, clonal, and sub-clonal PICs. Data are mean ± SD; bulk, n = 1; clones, n = 7; sub-clones, n = 3. e qRT-PCR transcript analysis of bulk, clones, and sub-clones. Bars represent the mean relative expression normalised to GAPDH. Error bars represent the standard deviation of the mean; n = 3

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Stem Cell Research & Therapy

ISSN: 1757-6512