Table 1 Antibodies selected for immunohistochemistry and flow cytometry characterization of equine MSCs
From: Isolation and characterization of equine endometrial mesenchymal stromal cells
Antibody | Host, isotype | Epitope | Clone | Company |
|---|---|---|---|---|
CD29 | Mouse, IgG1,κ | Human | TS2/16 | BioLegend |
CD44 | Mouse, IgG1 | Horse | CVS18 | AbD Serotec |
CD90 | Mouse, IgG1,κ | Rat | OX-7 | BD Pharmingen |
CD105 | Mouse, IgG1 | Human | SN6 | AbD Serotec |
NG2:APC | Mouse, IgG1 | Human | LHM-2 | R&D Systems |
CD146:FITC | Mouse, IgG1 | Human | OJ79c | AbD Serotec |
CD34:PE | Mouse, IgG1 | Human | 4H11[APG] | Immuno Tools |
MHC-II:FITC | Mouse, IgG1 | Horse | CVS20 | AbD Serotec |
Mucin-1 | Goat, IgG | Â | Polyclonal | Santa Cruz |
Secondary antibodies | ||||
 IgG—AF568 | Donkey | Mouse | Polyclonal | Invitrogen |
 IgG—AF488 | Goat | Mouse | Polyclonal | Invitrogen |
 IgG—AF568 | Donkey | Goat | Polyclonal | Invitrogen |
Isotype controls | ||||
 Mouse IgG1, κ |  |  | MOPC-21 | BioLegend |
 Mouse IgG1 |  |  | 11711 | R&D Systems |
 Mouse IgG1:APC |  |  | 11711 | R&D Systems |
 Mouse IgG1:PE |  |  | PPV-06 | Immuno Tools |
 Mouse IgG1:FITC |  |  |  | AbD Serotec |
 Goat IgG |  |  |  | Santa Cruz |