Table 2 Primers used for qPCR analysis
From: Isolation and characterization of equine endometrial mesenchymal stromal cells
Marker | Primer sequence (5′–3′) | ||
|---|---|---|---|
Reference gene | 18S | Forward | GCT GGC ACC AGA CTT G |
Reverse | GGG GAA TCA GGG TTC G | ||
TBP | Forward | CCA AGC GTT TTG CTG TA | |
Reverse | TTC ACT CTT GGC TCC CG | ||
GAPDH | Forward | GAA GAT GTG GCG CGA TGG CC | |
Reverse | ACT GAC ACG TTA GGG GTG GGG AC | ||
MSC marker | CD29 | Forward | GGC TAA CAG GGA GTT TCA GAT |
Reverse | ACA TCT ATT TTC ATC TGC TTG GC | ||
CD44 | Forward | CCC ACG GAT CTG AAA CAA GTG | |
Reverse | TTC TGG AAT TTG AGG TCT CCG TAT | ||
CD90 | Forward | TGC GAA CTC CGC CTC TCT | |
Reverse | GCT TAT GCC CTC GCA CTT G | ||
CD105 | Forward | GAC GGA AAA TGT GGT CAG TAA TGA | |
Reverse | GCG AGA GGC TCT CCG TGT T | ||
Perivascular marker | CD146 | Forward | CTG GAC TTG GAA ACC ACA ACA TC |
Reverse | CAG GTC TCA CTC GGA CAT CAG A | ||
NG2 | Forward | CGA ATC ATT GGG CCC TAC TT | |
Reverse | GCT GTT CCA CCT CTC TCC AG | ||
Hematopoietic marker | CD34 | Forward | CAC TAA ACC CTC TAC ATC ATT TTC TCC TA |
Reverse | GGC AGA TAC CTT GAG TCA ATT TCA | ||
CD45 | Forward | TGA TTC CCA GAA ATG ACC ATG TA | |
Reverse | ACA TTT TGG GCT TGT CCT GTA AC | ||
Epithelial marker | Mucin-1 | Forward | CTA TCT CGT TGC CCT GGC TG |
Reverse | GTA GGC ATC ACG GGT TGG AA | ||
Smooth muscle marker | ACTA2 | Forward | CTA ACA ACG TCC TCT CCG GG |
Reverse | CTG CTG GAA GGT GGA CAG AG | ||
CNN1 | Forward | CGG CAA CTT CAT GGA CG | |
Reverse | TTC TCC AGC TGG TGC CAA T | ||
MHY11 | Forward | ATC CAT CCT GAC CCC ACG TA | |
Reverse | CGG AAG AGC CGC TCA TAA GT | ||