Fig. 5

Teratoma assay of engrafted T-MPCs cells in vivo show no teratoma in mice. Schematic illustration of teratoma assay performed in NOD-SCID gamma (NSG) mice. a Cells are subcutaneously injected into the fat pat. Following 7 months of monitoring the mice, no teratoma tumors were formed. Fat pad was harvested for whole-mount immunostaining. Fluorescence microscope images of b green fluorescent protein (GFP) and c GFP combined with DAPI stain show MPC nuclei in the injected mouse fat pad. d Immunostaining assay for the human-specific antibody HSP27 (red) show that cells are engrafted and survived for months after, but did not form teratoma. e Same image as d, with DAPI to demonstrate nuclei. White line indicates the grafted cells in the injection area. T-MPC tonsillar mesenchymal progenitor cell