Fig. 4

Impaired capacity of CD106⁺/CD106^– BM-MSCs from aplastic anemia (AA) patients and controls to support colony formation of UCB CD34⁺ cells. Purified CD34⁺ cell-derived colony formation of burst-forming unit-erythroid (BFU-E) (A), colony-forming unit-granulocyte macrophage (CFU-GM) (B), CFU mixed cell (CFU-GEMM) (C), and CFU-megakaryocyte (CFU-MK) (D) on a layer of unsorted mesenchymal stem cells (UMSCs), CD106⁺ MSCs, or CD106^– MSCs from healthy controls (n = 5) and AA patients (n = 5). E The shape of CFUs. The CFU-GM, BFU-E, and CFU-GEMM colonies in the presence of unsorted MSCs, CD106⁺ MSCs, or CD106^– MSCs from healthy controls (a, b, and c) and from AA patients (d, e, and f). F Impaired capacity of BM-MSCs from AA patients to promote the CFU-MK formation of UCB CD34⁺ cells. The size of the CFU-MK colony in the presence of unsorted MSCs, CD106⁺ MSCs, or CD106^– MSCs from healthy controls (a, c, and e) was significantly larger than that of CFU-MK in the presence of the corresponding MSCs from AA patients (b, d, and f). Data are represented as the mean ± standard error. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001