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Fig. 5 | Stem Cell Research & Therapy

Fig. 5

From: Human dental pulp pluripotent-like stem cells promote wound healing and muscle regeneration

Fig. 5

DPPSC engraftment, differentiation and revascularisation in Sgcb-null Rag2-null γc-null dystrophic mice at day 20. a, b Immunofluorescence analysis for hLMNA (green) and laminin (red), showing DPPSC engraftment in Sgcb-null Rag2-null γc-null mice. Arrows indicate DPPSC in the interstitial space, while arrowheads indicate integration in the fibres. c Immunofluorescence analysis for hLMNA (red) and αSMA (green) in DPPSC-injected Sgcb-null Rag2-null γc-null muscles; a higher magnification of a human cell integrated in the SMC layer of a blood vessel is shown in the inset. d, e Immunofluorescence analysis for beta-sarcoglycan (SGCB; in red) in control (d) and DPPSC-injected (e) Sgcb-null Rag2-null γc-null muscles. f, g vWF (green) and αSMA (red) immunofluorescence analysis in control (f) or DPPSC-injected (g) Sgcb-null Rag2-null γc-null muscles, showing the presence of vWF+ vessels with αSMA coverage. For a-g, nuclei are counterstained with DAPI (blue); scale bars: 50 μm. h Quantitative analysis of the area of vWF+ or αSMA+ vessels per mm2 of tissue in Sgcb-null Rag2-null γc-null muscles. **p < 0.01, n = 4 mice/group. Two-tailed Student’s t test was used and results are displayed as mean ± s.e.m

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