Fig. 7
From: Extracellular vesicles from human liver stem cells restore argininosuccinate synthase deficiency
The expression of the ASS1 gene in HLSCs and EVs. ASS1 gene expression was determined in human liver stem cell-derived extracellular vesicles (HLSC-EVs) by PCR analysis. Two different regions of human ASS1 (CTLN1) gene located in exons 14 and 15 were amplified by PCR with two different primers that amplified both the regions respectively; exon 14 (Primer 1), exon 15 (Primer 2). A PCR without the presence of nucleotides was used as negative control (–C). Three different preparations of EVs (lanes 2, 3, and 4 and lanes 6, 7, and 8) were analyzed in comparison with the cells of origin (HLSCs; lanes 1 and 5)