Fig. 6

The osteogenic potential of pulpal (DMSCs) and gingival (GMSCs) mesenchymal stem cells. a A representative image of Alizarin Red assay stained calcium deposits after 15 days of osteogenic differentiation cultured in healthy (H)-DPSCs and H-GMSCs with or without cytokine (cyt) preconditioning, and periodontally affected (P)-DPSCs, P-GMSCs, and bone marrow MSCs (BM-MSCs) with or without cytokine preconditioning (control). b The bar graph represents the quantitative analysis of Alizarin by means of spectrometry (reading at 550 OD). c the bar graphs represent the relative mRNA expression of early and later osteogenic differentiation markers. Culture passage P5. *p < 0.05; **p < 0.01. FC fold change, n.s. not significant