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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: Protein deubiquitinase USP7 is required for osteogenic differentiation of human adipose-derived stem cells

Fig. 3

Overexpression of USP7 promotes osteogenic differentiation of hASCs in vitro. a Validation of USP7 overexpression effect by qRT-PCR and Western blotting, respectively. b Images of ALP staining on day 7, and AZR staining on day 14 in control or USP7 overexpression cells treated with proliferation or osteogenic media. c Histograms show ALP activity and quantification of AZR staining by spectrophotometry. d Relative mRNA expression of ALP and OC measured by qRT-PCR on day 14 of osteogenic induction. GAPDH was used for normalization. Results are presented as the mean ± SD, n = 3. *P < 0.05, **P < 0.01. ALP alkaline phosphatase, AZR alizarin red S, hASC human adipose-derived stem cell, OC osteocalcin, OM osteogenic media, PM proliferation media

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Stem Cell Research & Therapy

ISSN: 1757-6512