Source | Sample weight (g) | Nucleated cell number (×103 cells)/gram of tissue | Colony number/nucleated cells (CFU %) | Mean colony area (mm2) | P0 cMSC (×106)/plate |
|---|
Synovium | 0.24 ± 0.01 | 46.6 ± 62.80 | 6.48 ± 3.49 | 0.28 ± 0.03 | 3.13 ± 3.64 |
Marrow | 4.20 ± 0.80 | 18.70 ± 28.10 | 0.01 ± 0.01 | 0.26 ± 0.13 | 0.48 ± 0.4 |
Adipose | 0.31 ± 0.10 | 12.90 ± 12.00 | 2.63 ± 2.17 | 0.26 ± 0.13 | 2.76 ± 5.45 |
- All data reported as mean ± standard deviation
- Nucleated cells were isolated using Ficoll™ centrifugation (marrow) or enzymatic digestion (synovium, adipose) and plated at clonal density. Tissue sample weights and the number of nucleated cells recovered from tissue samples adjusted per gram of tissue are presented. After isolation, the colony forming unit (CFU) potential of primary cell populations for all 15 donors was performed: 1 × 103 total cells (synovium and adipose) or 4.5 × 105 total cells (marrow) were seeded on 55-cm2 plates and incubated for 21 days. Plates were stained with 0.3% crystal violet and the colony number and surface area (mm2) were determined for each plate. Using separate MSC isolation plates, the numbers of plastic-adherent canine multipotent stromal cells (cMSCs) recovered at passage 0 (P0) after 5–12 days in culture are reported as number of cMSCs/isolation plate
