Fig. 5

Differentiated iPSCs from an ADPKD patient and a healthy individual express different phenotypes. (a) Cell viabilities of TSG and TSB iPSCs tested by CCK-8 assays over the period from day 21 to day 28. Data presented as mean ± standard deviation from three independent sets of experiments, **P < 0.01. (b) Apoptosis rates of TSG and TSB iPSCs tested by Annexin V-FITC/PI staining over the period from day 21 to day 25. Data are averages of three independent experiments. (c) Water transportation assays carried out using induced TSG and TSB iPSCs. Data presented as mean ± standard deviation from three independent sets of experiments, **P < 0.01. (d) Marker genes of TSG and TSB iPSCs during the entire process of differentiating iPSCs to functional KLCs. Data presented as mean ± standard deviation from three independent sets of experiments. (e–g) BSA absorption assays of TSG and TSB KLCs derived from iPSCs. Bar = 25 μm. Data presented as mean ± standard deviation from three independent sets of experiments, **P < 0.01. ADPKD autosomal dominant polycystic kidney disease, TSB, TSG names of family members (Color figure online)