Generation of special autosomal dominant polycystic kidney disease iPSCs with the capability of functional kidney-like cell differentiation

Table 1 Primers for PKD1 and PKD2

Fragment	Size (kb)	Exons	Forward primer	Reverse primer	Temperature (°C)
PKD1
L1	2.2	1	CCATCCACCTGCTGTGTGACCTGGTAAAT	CCACCTCATCGCCCCTTCCTAAGCAT	68
L2	4.6	2–7	ATTTTTTGAGATGGAGCTTCACTCTTGCAGG	CGCTCGGCAGGCCCCTAACC	68
L3	4.2	8–12	CCGCCCCCAGGAGCCTAGACG	CATCCTGTTCATCCGCTCCACGGTTAC	68
L4	4.4	13–15	TGGAGGGAGGGACGCCAATC	GTCAACGTGGGCCTCCAAGT	68
L5	3.4	15–21	AGCGCAACTACTTGGAGGCCC	GCAGGGTGAGCAGGTGGGGCCATCCTA	70
L6	0.3	22	GAGGCTGTGGGGGTCCAGTCAAGTGG	AGGGAGGCAGAGGAAAGGGCCGAAC	64
L7	4.2	23–28	CCCCGTCCTCCCCGTCCTTTTGTC	AAGCGCAAAAGGGCTGCGTCG	68
L8	5.8	29–34	GGCCCTCCCTGCCTTCTAGGCG	GTTGCAGCCAAGCCCATGTTA	68
35–37	0.7	35–37	GGGATGAATTCACAGCCTAC	GGAGACAAGAGACGGAGGT	62
38–40	1.1	38–40	AAGCCCTGCTGTCACTGT	TACTCCCTTGTCCTTGGC	56
41–43	1.1	41–43	GGGAGTAGTTCTCCAGGAGTG	CGAGAAATCTGTCTGCTTGC	62
44–46	1.1	44–46	GGCTGCAAGCAGACAGATT	GCGGTGTCCACTCCGACTCC	56
PKD2
1-1	0.5	1	AGAGGGAGGCGGGCCAAAGG	CGGGCGCCACTCTACGTCCA	62
1-2	0.4	1	GTGGAGCCGCGATAACCCCG	AGGCGGAACGCAGAGGGGAT	62
2	0.5	2	TTGTGCTTTATTTTCCCTTTTGCCA	TGCCTCTCCCGTCCTGTGTT	59
3–4	2.5	3–4	AGGGGAAAGGAAGGCAAGGGTGA	TGCCTTGGTGAAGGTGTCAGGGA	65
5–6	4.0	5–6	GCCAGGTCAGGCACAGTACCC	AGCGTGGCTGAGAGCATACTGT	63
7–8	4.5	7–8	TGGCAGGGCTTAACACTTTCCATTT	TCTTGAGAAGCAGTGACAACTCTGA	65
9–10	4.7	9–10	ACCGTGCCCAGCTTGTGTTT	CTGCCGTGGAAGGTCAAGGG	65
11–13	2.9	11–13	CCAGCACGTACTTGTTGAATGGCC	GGGAACTGCCTGGTCTCATGTGG	65
14–15	1.0	14–15	GCCAGTGGGGCTGAAAAGACA	AGCATCCTATGGTGGTCAGGGCA	70

ISSN: 1757-6512