Table 3 Primers for characterization
Gene | Forward sequence | Reverse sequence |
|---|---|---|
ACTB | CCCAGAGCAAGAGAGG | GTCCAGACGCAGGATG |
endo-OCT4 | CCTCACTTCACTGCACTGTA | CAGGTTTTCTTTCCCTAGCT |
endo-Sox2 | CCCAGCAGACTTCACATGT | CCTCCCATTTCCCTCGTTTT |
Nanog | TGAACCTCAGCTACAAACAG | TGGTGGTAGGAAGAGTAAAG |
Rex1 | TCGCTGAGCTGAAACAAATG | CCCTTCTTGAAGGTTTACAC |
AFP | ATTGGCAAAGCGAAGCTG | GCTGTGGCTGCCATTTTT |
CK18 | AGCTCA ACGGGATCCTGCTGCACCTTG | CACTATCCGGCGGGGGTGGCTTTTG |
MSX1 | CACTATCCGGCGGGGGTGGCTTTTG | CGAGAGGACCCCGTGGATGCAGAG |
TBX1 | AGCGAGAAATATGCCGAGG | TTCGCGAAGGGATTGCT |
PAX6 | TTCGCGAAGGGATTGCT | TGCCCGTTCAACATCCTT |
SOX1 | TTTCCCCTCGCTTTCTCA | TGCAGGCTGAATTCGGTT |
PAX2 | AGATTCCCAGAGTGGTGTG | GGGTATGTCTGTGTGCCTGA |