Fig. 6

Morphology and growth of sorted CD264^– and CD264⁺ MSCs. P6 MSCs from a female donor (age 52 years) were surface labeled with anti-CD264-phycoerythrin (PE) and sorted into CD264-negative and CD264-positive groups. a,b Histograms depict MSCs before (a) and after (b) cell sorting (n = 10,000 cells/group). c,d Scatter properties of the sorted cell groups. e,f Representative phase-contrast micrographs of the sorted MSCs. Arrows point to enlarged cells with flattened, granular cytoplasm. Scale bars = 100 μm. g,h Growth curve of CD264^– (circle) and CD264⁺ (triangle) cultures (g) and corresponding doubling times (h). Data are reported as mean ± SEM for n = 3 biological replicates. *p < 0.05, **p < 0.01, versus CD264^– MSCs