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Fig. 3 | Stem Cell Research & Therapy

Fig. 3

From: miR-524-5p of the primate-specific C19MC miRNA cluster targets TP53IPN1- and EMT-associated genes to regulate cellular reprogramming

Fig. 3

Inverse relationship between expression of miR-524-5p and TP53INP1. a Expression of TP53INP1 in different cell lines determined by 25 cycles of RT-PCR. iPSC (MH#1) and iPSC (ASC-iPSC) were derived from the adipose-MSC cell lines, ASC Lonza and ASC-Inv, respectively. ESC (H9), placenta (HS799.PI), colon cancer (HCT-15), and breast cancer (MCF-7) cell lines were included for comparison and as controls. b Efficient transfection and overexpression of the miR-524-5p mimic in HCT-15 cells. c,d Effects of miR-524-5p overexpression on TP53INP1 expression. A miR-524-5p mimic was transfected into HCT-15 cells for 48 h before the cells were harvested for 40 cycles of real-time RT-PCR (c) or Western blot analysis (d) to determine TP53INP1 expression. As a control, a TP53INP1 siRNA (siTP53INP1) was also used in the transfection. *p < 0.05, relative to mock transfection. ASC adipose-derived stem cell, ESC embryonic stem cell, iPSC induced pluripotent stem cell, MSC mesenchymal stem cell, NC negative control

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Stem Cell Research & Therapy

ISSN: 1757-6512