Fig. 5

Immunofluorescence staining of netrin-1. The upper panels show the expression of netrin-1 in different areas of the brain at day 14 after MCAO. Netrin-1 was mainly expressed in the peri-infarct cortex (a), whereas weak immunoreactivity was found in the contralateral cerebral cortex (b), and no visible immunoreactivity was observed in the infarct core (c). The lower three panels show the dual-labeled immunofluorescence for netrin-1 (red) and neuron-specific enolase (NSE; green) (d), netrin-1 and glial fibrillary acidic protein (GFAP; green) (e), and netrin-1 and von Willebrand factor (vWF; green) (f) in the peri-infract cortex at day 14 after MCAO, respectively, and nuclei were stained with 4,6-diamidino-2-phenylindole (DAPI; blue). Netrin-1 was mainly expressed in NSE⁺ neuronal perikarya, but did not colocalize with GFAP⁺ astrocytes and vWF⁺ blood vessels. Scale bar = 50 μm