Fig. 6

Immunofluorescence staining of DCC. The upper panels show the expression of deleted in colorectal cancer (DCC) in different areas of the brain at day 14 after MCAO. Immunoreactivity for DCC was predominantly found in the peri-infarct cortex (a), with little immunoreactivity in the contralateral cerebral cortex (b) and no visible immunoreactivity in the infarct core (c). The lower three panels show the dual-labeled immunofluorescence for DCC (red) and neurofilament-200 (NF-200; green) (d), DCC and glial fibrillary acidic protein (GFAP; green) (e), and DCC and von Willebrand factor (vWF; green) (f) in the peri-infract cortex at day 14 after MCAO, respectively, and nuclei were stained with 4,6-diamidino-2-phenylindole (DAPI; blue). DCC mainly colocalized with NF-200⁺ neuronal fibers, and sparsely with GFAP⁺ astrocytes. Though DCC did not colocalize with vWF⁺ blood vessels, obvious DCC immunoreactivity was evident around the vWF⁺ blood vessels (white arrowheads). Scale bar = 50 μm