Fig. 1

Omentin-1 activates the PI3K/Akt signaling pathway in a concentration- and time-dependent manner. The activation of the PI3K/Akt pathway was evaluated by Western blotting. a,b MSCs were incubated with different concentrations of omentin-1 (0–800 ng/ml) in serum-free medium for 1 h, or LY294002 (20 μm/L) was added to the MSC medium for 1 h before omentin-1 (800 ng/ml) treatment. c,d MSCs were treated with omentin-1 (800 ng/ml) in serum-free medium for the indicated periods (0–120 min). e,f Omentin-1 induced FoxO3a and GSK-3β phosphorylation via the PI3K/Akt pathway in MSCs. Data are presented as the mean ± SD of three separate experiments. *P < 0.05, versus the control group; ^# P < 0.05, versus the 800 ng/ml omentin-1 treated group. Cont. control, LY LY294002, p-Akt phosphorylated Akt (Ser473 and Thr308), p-FoxO3a phosphorylated FoxO3a, p-GSK-3β phosphorylated GSK-3β, t-Akt total Akt, t-FoxO3a total FoxO3a, t-GSK-3β total GSK-3β