Fig. 2

Adjudin pretreatment attenuated cell death and maintained the ATP level of NSCs after H₂O₂ stimulation. Assays to evaluate whether adjudin pretreatment could attenuate cell death of NSCs after 0.1 mM H₂O₂ exposure. Cell death and cell survival measured by LDH (a) and CCK-8 assay (b). ATP level of NSCs after H₂O₂ stimulation with/without adjudin pretreatment (c). Levels of p-Akt and Akt detected by western blot analysis after H₂O₂ treatment (d). Quantification of the densitometric value of protein bands normalized to total Akt (e). Bars represent mean ± SEM from three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001. LDH lactate dehydrogenase