Fig. 7

vWF immunohistochemistry in mouse lung tissue at PND7 and PND14 (n = 6–8). a Representative images for vWF staining on PND14. Scale bar = 100 μm. b, c Average number of vessels per field of view on PND7 (b) and PND14 (c). Average number of vessels with diameter < 50 μm was decreased in saline-treated injured mice, which was reversed after hAEC treatment on both PND7 and PND14. Black bars refer to vessels < 50 μm and grey bars refer to vessels > 50 μm. There was no difference in the numbers of larger blood vessels (i.e. > 50 μm) between groups. Data expressed as mean ± standard error of mean (SEM). Statistical significance determined with one-way ANOVA accompanied by the Bonferroni post-hoc test. **p < 0.01, ****p < 0.0001. hAEC human amnion epithelial cell, PND postnatal day