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Table 2 qPCR validation of differentially expressed miRNAs

From: miRNA profiling of NurOwn®: mesenchymal stem cells secreting neurotrophic factors

miRNA ID Microarray fold change
(MSC-NTF vs MSC)
Function qPCR validated
(p ≤ 0.05)
Fold change
(MSC-NTF vs MSC)
hsa-miR-503-5p −8.38 Anti-angiogenic [17, 25] Promotes osteogenesis [26] (Originates from the same primary RNA transcript as miR-424 [27] Yes −30.5
hsa-miR-320b −2.41 Involved in neural development, anti-angiogenic [28] Yes −1.36
hsa-miR-320a −1.97 Targets VEGF A [16] No −1.28
hsa-miR-222-3p −1.74 Negative modulator of angiogenesis Inhibition of osteogenic differentiation [29] No −1.25
hsa-miR-424-5p −1.72 Anti-angiogenic [25] Targets VEGF A, VEGFR-2 and FGF2 [15] Yes −1.94
hsa-miR-3663-3p 8.03 On/off signal. No validated mRNA target Yes 18.5
hsa-miR-132-3p 7.85 Pro-angiogenic [30] Inhibits osteoblast differentiation [31] Regulation of synaptic structure [18] Yes 4.06
hsa-miR-150-3p 6.99 On/off signal. Inhibits osteoblast differentiation downstream of TNF-α [32] Not validated but significantly downregulated −4.44
hsa-miR-762 5.91 Upregulated in neural precursors [33] No 1.47
hsa-miR-34a-5p 4.05 Tumor suppressor: pro-apoptotic and anti-proliferative [34] TNF-α suppressor [35] Inhibits osteoblast differentiation [36] Yes 3.16
  1. miRNA microRNAs, MSC mesenchymal stromal cells, NTF neurotrophic factors, VEGF(R) vascular endothelial growth factor (receptor), FGF fibroblast growth factor, TNF-α tumor necrosis factor alpha