miRNA profiling of NurOwn®: mesenchymal stem cells secreting neurotrophic factors

Table 2 qPCR validation of differentially expressed miRNAs

miRNA ID	Microarray fold change (MSC-NTF vs MSC)	Function	qPCR validated (p ≤ 0.05)	Fold change (MSC-NTF vs MSC)
hsa-miR-503-5p	−8.38	Anti-angiogenic [17, 25] Promotes osteogenesis [26] (Originates from the same primary RNA transcript as miR-424 [27]	Yes	−30.5
hsa-miR-320b	−2.41	Involved in neural development, anti-angiogenic [28]	Yes	−1.36
hsa-miR-320a	−1.97	Targets VEGF A [16]	No	−1.28
hsa-miR-222-3p	−1.74	Negative modulator of angiogenesis Inhibition of osteogenic differentiation [29]	No	−1.25
hsa-miR-424-5p	−1.72	Anti-angiogenic [25] Targets VEGF A, VEGFR-2 and FGF2 [15]	Yes	−1.94
hsa-miR-3663-3p	8.03	On/off signal. No validated mRNA target	Yes	18.5
hsa-miR-132-3p	7.85	Pro-angiogenic [30] Inhibits osteoblast differentiation [31] Regulation of synaptic structure [18]	Yes	4.06
hsa-miR-150-3p	6.99	On/off signal. Inhibits osteoblast differentiation downstream of TNF-α [32]	Not validated but significantly downregulated	−4.44
hsa-miR-762	5.91	Upregulated in neural precursors [33]	No	1.47
hsa-miR-34a-5p	4.05	Tumor suppressor: pro-apoptotic and anti-proliferative [34] TNF-α suppressor [35] Inhibits osteoblast differentiation [36]	Yes	3.16

miRNA microRNAs, MSC mesenchymal stromal cells, NTF neurotrophic factors, VEGF(R) vascular endothelial growth factor (receptor), FGF fibroblast growth factor, TNF-α tumor necrosis factor alpha

ISSN: 1757-6512