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Table 1 Induction efficiency and characterization of iPSCs induced from hUCs using the 6F/BM1-4C system

From: Using low-risk factors to generate non-integrated human induced pluripotent stem cells from urine-derived cells

Donor Age Gender Cell number Death ratea (%) Efficiency (%) No. Non-integration Karyotype FACS BSP Teratomab EB formation Immunofluorescent
1 23 Female 3 × 106 53.8 0.0468 UC1iPSC1 +c + + + + + +
UC1iPSC2 +c + +
2 26 Male 2.8 × 106 80.37 0.0015 UC2iPSC1 +c + + + + +
UC2iPSC2 +c +   +
3 27 Male 3 × 106 50 0.0741 UC3iPSC1 +c + + +
UC3iPSC2 +c + +
4 26 Female 3 × 106 71 0.00089 UC4iPSC1 +c +
UC4iPSC2 +c + +e
5 24 Male 3 × 106 66.35 0.00095 UC5iPSC1 +c + +
UC5iPSC2 +c + +e
6 30 Female 2.53 × 106 36 0.00021 UC6iPSC1 +d +
UC6iPSC2 +c + +
7 29 Male 3 × 106 27.5 0.0429 UC7iPSC1 +c + +e
UC7iPSC2 +c +
  1. At nucleofection, the hUC passage number was 2
  2. + iPSCs identified, – characterization not identified, iPSC induced pluripotent stem cell, hUC human urine-derived cell, FACS fluorescence-activated cell sorting, EB embryoid body, HE hematoxylin and eosin
  3. aTaking supernatant (containing nonadherent cells) to count cells on the 3rd day after electronic transformation, the amount of counted cells was the amount of dead cells. Death rate calculated as: death rate = amount of dead cells/amount of cells for electroporation
  4. bWe injected five different donor-derived iPSCs in the teratoma experiment. Each NOD/SCID mouse was injected with a cell. In 6–8 weeks, three NOD/SCID mice formed teratomas and were dissected and HE stained. In the 8th week, one NOD/SCID mice had granule-shaped teratoma, and the teratoma was taken out and HE stained in the 12th week. One mouse did not form teratoma by observation
  5. cExogenous gene sequence did not integrate in the genome
  6. dExogenous gene sequence integrated in the genome
  7. eCorresponding primary cells did not detect the pluripotency gene promoter DNA methylation due to the exhausted cells