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Table 1 Induction efficiency and characterization of iPSCs induced from hUCs using the 6F/BM1-4C system

From: Using low-risk factors to generate non-integrated human induced pluripotent stem cells from urine-derived cells

Donor

Age

Gender

Cell number

Death ratea (%)

Efficiency (%)

No.

Non-integration

Karyotype

FACS

BSP

Teratomab

EB formation

Immunofluorescent

1

23

Female

3 × 106

53.8

0.0468

UC1iPSC1

+c

+

+

+

+

+

+

UC1iPSC2

+c

+

+

2

26

Male

2.8 × 106

80.37

0.0015

UC2iPSC1

+c

+

+

+

+

+

UC2iPSC2

+c

+

 

+

3

27

Male

3 × 106

50

0.0741

UC3iPSC1

+c

+

+

+

UC3iPSC2

+c

+

+

4

26

Female

3 × 106

71

0.00089

UC4iPSC1

+c

+

UC4iPSC2

+c

+

+e

5

24

Male

3 × 106

66.35

0.00095

UC5iPSC1

+c

+

+

UC5iPSC2

+c

+

+e

6

30

Female

2.53 × 106

36

0.00021

UC6iPSC1

+d

+

UC6iPSC2

+c

+

+

7

29

Male

3 × 106

27.5

0.0429

UC7iPSC1

+c

+

+e

UC7iPSC2

+c

+

  1. At nucleofection, the hUC passage number was 2
  2. + iPSCs identified, – characterization not identified, iPSC induced pluripotent stem cell, hUC human urine-derived cell, FACS fluorescence-activated cell sorting, EB embryoid body, HE hematoxylin and eosin
  3. aTaking supernatant (containing nonadherent cells) to count cells on the 3rd day after electronic transformation, the amount of counted cells was the amount of dead cells. Death rate calculated as: death rate = amount of dead cells/amount of cells for electroporation
  4. bWe injected five different donor-derived iPSCs in the teratoma experiment. Each NOD/SCID mouse was injected with a cell. In 6–8 weeks, three NOD/SCID mice formed teratomas and were dissected and HE stained. In the 8th week, one NOD/SCID mice had granule-shaped teratoma, and the teratoma was taken out and HE stained in the 12th week. One mouse did not form teratoma by observation
  5. cExogenous gene sequence did not integrate in the genome
  6. dExogenous gene sequence integrated in the genome
  7. eCorresponding primary cells did not detect the pluripotency gene promoter DNA methylation due to the exhausted cells