Fig. 4

Immunofluorescence staining of WJ-MSC differentiation into ESC-like cells in the four groups: (a) untreated WJ-MSCs; (b) WJ-MSCs treated with 8-Br-CAMP and E2; (c) WJ-MSCs treated with 8-Br-CAMP; (d) WJ-MSCs treated with E2. (A) Cells in the four groups stained with anti-vimentin antibodies (green), anti-CD13 (red), and the Hoechst nuclear dye (blue). Merged images displayed in the fourth column. (B) Cells in the four groups stained with anti-cytokeratin antibodies (green), anti-CD9 (red), and the Hoechst nuclear dye (blue). Merged images displayed in the fourth column. Bar represents 200 μm