Fig. 2

Hepatocyte differentiation from hepatic progenitor cells was affected by Cxs expression. a Overexpression of Cx32 and Cx43, and knockdown of Cx43 in WB cells were confirmed by Western blotting. b qRT-PCR analysis of hepatic markers in cells differentiated from WB cells with Cx32 or Cx43 overexpression, or Cx43 knockdown. c Western blotting of hepatic markers in cells differentiated from WB cells with Cx32 or Cx43 overexpression, or Cx43 knockdown. d ICG uptake. e Urea production of WB cells with Cx32 or Cx43 overexpression, 2APB, or Cx43 knockdown. Scale bars: 100 μm. Data represented as mean ± SEM. ^* p < 0.05, ^** p < 0.01