Fig. 3

miR-200c reduces the MALAT1 level. a, b qRT-PCR showing relative RNA levels of XIST (a) and MALAT1 (b) in 12 EC and eight EN tissues. c Pearson correlation analysis of miR-200c and MALAT1 RNA levels in EC tissues represented by Pearson R scores (n = 27, R < 0 denotes negative correlation). d Schematic representation of wild-type and mutant pMIR-REPORT-MALAT1 miRNA expression vectors used in luciferase reporter assays. Three nucleotides altered in the mutant binding site are colored red. e, f Relative luciferase activities in HESCs corresponding to binding site 1 (e) and binding site 2 (f) after cotransfection with the pMIR-REPORT-MALAT1 reporter and the miR-200c mimic (50 nM), mimic NC, miR-200c inhibitor (100 nM), or inhibitor NC for 48 hours. g qRT-PCR analysis of MALAT1 expression in HESCs after transfection with the miR-200c mimic (50 nM), mimic NC, miR-200c inhibitor (100 nM), or inhibitor NC for 24 hours. h, i Relative RNA levels of MALAT1 (h) and miR-200c (i) in HESCs that were transiently transfected with siMALAT1(50 nM) or siNC for 24 hours. All data shown as mean ± SD of three independent experiments (*P < 0.05 and **P < 0.01, Student’s t test). EC ectopic endometrial, EN normal endometrial, MALAT1 metastasis-associated lung adenocarcinoma transcript 1, Mimic NC negative control miRNA mimic, Inhibitor NC negative control miRNA inhibitor, siRNA small interfering RNA, siRNA NC negative control siRNA