Fig. 1

Rabbit LESCs cultured from the three culture methods. a Tissue explants on the plate. b Corneal epithelial-like cells migrated from tissue explants after 3 days of culture. c, d Corneal epithelial-like cells near the tissue explants. e Tissue explants became loose after digestion with dispase for 18 hours. f–h Small epithelial-like cells extended, fused, and generated epithelial sheet. i, j Tissue explants were subjected to collagenase digestion in KSFM for 18 hours to yield cell clusters. k, l Clusters grew like colonies with LESCs in the middle. Scale bar, 50 μm