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Fig. 1 | Stem Cell Research & Therapy

Fig. 1

From: Poly(ethylene glycol)-modified silk fibroin membrane as a carrier for limbal epithelial stem cell transplantation in a rabbit LSCD model

Fig. 1

Rabbit LESCs cultured from the three culture methods. a Tissue explants on the plate. b Corneal epithelial-like cells migrated from tissue explants after 3 days of culture. c, d Corneal epithelial-like cells near the tissue explants. e Tissue explants became loose after digestion with dispase for 18 hours. fh Small epithelial-like cells extended, fused, and generated epithelial sheet. i, j Tissue explants were subjected to collagenase digestion in KSFM for 18 hours to yield cell clusters. k, l Clusters grew like colonies with LESCs in the middle. Scale bar, 50 μm

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