Fig. 6

Rabbit LESCs cultured on PEG-modified SF membranes maintain normal corneal differentiation. a, b Corneal epithelial differentiation marker CK12 and conjunctival epithelial cell marker CK7 staining of LESCs cultured on PEG-modified SF membranes for 15 days. Percentages of CK12⁺ corneal epithelial cells and CK7⁺ conjunctival epithelial cells on PEG-modified SF membranes were quantified. Data was shown as mean ± SD from three independent experiments. Student’s t test: ***P < 0.001. c, d LESCs cultured on PEG-modified SF membranes generated corneal epithelial tight junctions (Claudin-1 staining). Scale bar, 50 μm. CK7/12 cytokeratin7/12, DAPI 4′,6-diamidino-2-phenylindole